Biography:

Wei Dai has completed her Master’s degree from Anhui Agricultural University, China. She is a Doctor of the State Key Laboratory of Bioelectronics, Southeast University, China. She has published several papers in reputed journals with impact factor more than 15.

 

 

Abstract:

It has been well-known that over activation of NF-кB has close relationship with hepatitis and hepatocellular carcinoma (HCC). However, the complete and exact underlying molecular pathways and mechanisms still remain not fully understood. By manipulating NF-кB activity with its recognized activator TNFα and using ChIP-seq and RNA-seq techniques, this study identified 699 NF-кB direct target genes (DTGs) in a widely used HCC cell line, HepG2, including 399 activated and 300 repressed genes. In these NF-кB DTGs, 216 genes (126 activated and 90 repressed genes) are among the current HCC gene signature. In comparison with NF-кB target genes identified in LPS-induced THP-1 and TNFα-induced HeLa cells, only limited numbers (24~46) of genes were shared by the two cell lines, indicating the HCC specificity of identified genes. Functional annotation revealed that NF-кB DTGs in HepG2 cell are mainly related with many typical NF-кB-related biological processes including immune system process, response to stress, response to stimulus, defense response and cell death and signaling pathways of MAPK, TNF, TGF-β, chemokine, NF-кB and toll-like receptor. Some NF-кB DTGs are also involved in hepatitis C and B pathways. It was found that 82 NF-кB DTGs code secretory proteins, which include CCL2 and DKK1 that have already been used as HCC markers. Finally, the NF-кB DTGs were further confirmed by detecting the NF-кB binding and expression of 14 genes with ChIP-PCR and RT-PCR. This study thus provides a useful NF-кB DTG list for future studies of NF-кB-related molecular mechanisms and theranostic biomarkers of HCC.

 

Biography:

Salah Aref has completed his PhD from Mansoura University and Postdoctoral studies from Mansoura University, School of Medicine. He is the Director of Mansoutra University Oncology Center laboratories. He has published more than 60 papers in reputed journals and has been serving as an Editorial-Board Member of reputed journals

Abstract:

Salah Aref has completed his PhD from Mansoura University and Postdoctoral studies from Mansoura University, School of Medicine. He is the Director of Mansoutra University Oncology Center laboratories. He has published more than 60 papers in reputed journals and has been serving as an Editorial-Board Member of reputed journals

Biography:

Bela Zutshi has completed her PhD from Bangalore University, India. She is the Head of Department of Zoology, Bangalore University. She has served as a Member of State Level Environmental Assessment Committee, Government of Karnataka, India (2011-13). She has published more than 25 papers in reputed journals and written a book on status of lakes in Bangalore. She has handled two major research projects from UGC on fish toxicology and a minor project on biodiversity of blackbuck. She is Life Member of academic scientific bodies such as Asian Fisheries Society; Indian Science Congress; NEA; NESA and Aquatic Biodiversity Conservation Society, India.

 

Abstract:

 

Hematological study is important in toxicological research because hematological alteration is a good method for rapid evaluation of the chronic toxicities of a compound. Blood parameters are useful for the measurement of physiological disturbances in stressed fish and thus provide information about the level of damage in the fish. Genotoxic effect in erythrocytes of blood from fish, Labeo rohita reared for commercial purpose, was sampled from control site, Vengaiah Lake (A) and Yellamallappa Lake (B) during winter, summer and rainy seasons for a period of 24 months. The data of physicochemical parameters of water revealed high level of chemical oxygen demand, biological oxygen demand, turbidity, alkalinity and of trace metal content such as aluminium, cadmium, copper etc. and other pollutants in lake B (during all seasons) when compared to lake A, control site. Erythrocytic abnormalities including nuclear abnormalities (blebled, notched, eight shaped) and cytoplasmic variations (ruptured and vacuolated cytoplasm) were frequent in blood samples of fish from Lake B during winter. These results might be due to the discharge of treated/untreated industrial effluents from the Pharma industry present on the banks of Lake B and the agricultural runoff, idol immersion during festival season and discharge of domestic sewage and solid waste through various sources into the water body. The present study showed that high levels of chemical parameters and pollutants in both the lakes did not induce micronuclei but induced variation in frequency of nuclear and cytoplasmic abnormalities from summer to winter and rainy to be considered indicators of genotoxic damage.

 

Biography:

Adeyemi A Abati has completed his MBBS in 2004 at Obafemi Awolowo University Teaching Hospital, Nigeria. He was trained at the Department of Infectious Diseases during his Residency. He has completed his Master’s degree in Public Health from the same institution and currently, he is a PhD candidate. He currently holds a Certification from Nigerian Board of Internal Medicine for Internal Medicine, Hematology and Infectious Disease and awarded with Developing Leader in Medicine from Nigerian Medical Association in 2010 for his excellent contribution in general treatment and towards the reduction of infectious disease in Nigeria.

 

Abstract:

Introduction & Aim: In Nigeria, hepatitis C virus (HCV) infection is primarily spread through injection drug use. There is an urgent need to improve access to care for HCV among persons with opioid use disorders who inject drugs. The purpose of our study was to determine the prevalence of HCV, patient characteristics and receipt of appropriate care in a sample of patients treated with buprenorphine for their opioid use disorders in a primary care setting.
Method: This study used retrospective clinical data from the electronic medical record. The study population included patients receiving buprenorphine in the office based opioid treatment (OBOT) clinic within the adult primary medicine clinic at Lagos Medical Centre between October 2008 and August 2015, who received a conclusive HCV AB antibody test within a year of clinic entry. We compared characteristics by HCV serostatus using Pearson's chi-square and provided numbers/percentages receiving appropriate care.

Result: The sample comprised of 300 patients. Slightly less than half of all patients (n=134, 27.7%) were HCV ab positive and were significantly more likely to be older Hausas and Yoruba’s, have diagnoses of post-traumatic stress disorder (PTSD) and bipolar disorder, have prior heroin or cocaine use and be negatively infected. Among the 134 HCV ab positive patients, 126 (67.7%) had detectable HCV ribonucleic acid (RNA) indicating chronic HCV infection; only 8 patients (2.21%) with chronic HCV infection ever initiated treatment.

Conclusion: Nearly half of patients (47.7%) receiving office-based treatment with buprenorphine for their opioid use disorder had a positive hepatitis C virus antibody screening test, although initiation of HCV treatment was nearly non-existent (2.21%).

 

Biography:

Shiow-Kang Yen has completed his PhD from National Tsing Hua University, Taiwan. He was the Director of Department of Materials Science and Engineering, National Chung Hsing University. He was awarded with the Outstanding Researcher award at National Chung Hsing University in 2004. He has published more than 70 papers in reputed journals.

 

Abstract:

To reduce the side effects of chemotherapy, target therapies have been spotlighted. In this study, Cisplatin, the drug for cancer treatment, is electrochemically deposited on titanium alloy as vascular stents for the tumor localized therapy by sustaining drug releasing to achieve the cancer cells apoptosis or the prevention of cancer metastasis. Coupled with quartz crystal microbalance, the cathodic polarization tests were conducted to speculate the deposition mechanism of Cisplatin on titanium, the field emission scanning electron microscope and Fourier transform infrared spectroscopy to observe the surface morphology and analyze constituent elements, respectively. A UV visible spectrometer was used to measure drug loading and releasing. It is concluded that the Cisplatin can be successfully deposited on titanium with drug 62.99 μg/cm², by exchanging Cl^- ions which is originally bonded on Cisplatin with OH^- ions, is produced by the electrochemical method, finally to form strong hydrogen bonds and attract one another.

 

 

Biography:

Sadaf Batool currently working as a Resident Physician at Shaukat Khanum Memorial Cancer Hospital and Research Centre Balochisan, Pakistan. She completed her education from International Islamic University Islamabad, Pakistan and also has done Internship at ISSRA Pakistan.

 

 

Abstract:

Introduction & Objective: Hartmann’s procedure is normally performed for left sided colonic pathologies in emergency situations. Restoration of intestinal continuity after Hartmann’s procedure has traditionally been viewed to be technically demanding and associated with significant morbidity and mortality. This study has been done to show reversal rate after Hartmann’s procedure in an Asian population.
Method: Data collected from database showed that 105 patients had undergone Hartmann’s procedure from Jan 2006 to Dec 2015 due to colorectal carcinoma. Patients who subsequently underwent Hartmann’s reversal were identified and their records reviewed retrospectively.
Result: Hartmann’s procedure was done under emergency in 81 patients either due obstruction (65.1%), perforation (9.4%) and anastomotic leak (1.9%). It is done electively in 25 patients mostly due to poor bowel preparation secondary to stenosing nature of tumor. Hartmann’s reversal was done in 56 (52.8%) patients. The reversal was not offered in remaining patients either due to disease recurrence (36.7%), metastasis (26.5%), loss of follow up (28.5%) or others (8.1%).The median interval between resection and reversal was 32 weeks.
Conclusion: In our population, Hartmann’s procedure is more commonly performed for colorectal cancer under emergency situations. Reversal rate is 52.8% and the most common reasons for not reversing the disease are either locoregional recurrence or distant metastasis.